Impacts of Glutaraldehyde on Microbial Community Structure and Degradation Potential in Streams Impacted by Hydraulic Fracturing
| العنوان: | Impacts of Glutaraldehyde on Microbial Community Structure and Degradation Potential in Streams Impacted by Hydraulic Fracturing |
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| المؤلفون: | Campa, MF, Techtmann, SM, Gibson, CM, Zhu, X, Patterson, M, Garcia De Matos Amaral, A, Ulrich, N, Campagna, SR, Grant, CJ, Lamendella, R, Hazen, TC |
| المصدر: | Environmental science & technology, vol 52, iss 10 Campa, MF; Techtmann, SM; Gibson, CM; Zhu, X; Patterson, M; Garcia De Matos Amaral, A; et al.(2018). Impacts of Glutaraldehyde on Microbial Community Structure and Degradation Potential in Streams Impacted by Hydraulic Fracturing. Environmental Science and Technology, 52(10), 5989-5999. doi: 10.1021/acs.est.8b00239. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/4gg4v59f |
| بيانات النشر: | eScholarship, University of California, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Ribosomal, 16S, Rivers, Glutaral, Hydraulic Fracking, Microbiota, RNA, Pennsylvania, Environmental Sciences |
| الوصف: | © 2018 American Chemical Society. The environmental impacts of hydraulic fracturing, particularly those of surface spills in aquatic ecosystems, are not fully understood. The goals of this study were to (1) understand the effect of previous exposure to hydraulic fracturing fluids on aquatic microbial community structure and (2) examine the impacts exposure has on biodegradation potential of the biocide glutaraldehyde. Microcosms were constructed from hydraulic fracturing-impacted and nonhydraulic fracturing-impacted streamwater within the Marcellus shale region in Pennsylvania. Microcosms were amended with glutaraldehyde and incubated aerobically for 56 days. Microbial community adaptation to glutaraldehyde was monitored using 16S rRNA gene amplicon sequencing and quantification by qPCR. Abiotic and biotic glutaraldehyde degradation was measured using ultra-performance liquid chromatography - high resolution mass spectrometry and total organic carbon. It was found that nonhydraulic fracturing-impacted microcosms biodegraded glutaraldehyde faster than the hydraulic fracturing-impacted microcosms, showing a decrease in degradation potential after exposure to hydraulic fracturing activity. Hydraulic fracturing-impacted microcosms showed higher richness after glutaraldehyde exposure compared to unimpacted streams, indicating an increased tolerance to glutaraldehyde in hydraulic fracturing impacted streams. Beta diversity and differential abundance analysis of sequence count data showed different bacterial enrichment for hydraulic fracturing-impacted and nonhydraulic fracturing-impacted microcosms after glutaraldehyde addition. These findings demonstrated a lasting effect on microbial community structure and glutaraldehyde degradation potential in streams impacted by hydraulic fracturing operations. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::9eacc2ccdc605c6303d3356476f8e6d9 https://escholarship.org/uc/item/4gg4v59f |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.dedup.wf.001..9eacc2ccdc605c6303d3356476f8e6d9 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |