A New Approach for Designing a Potentially Vaccine Candidate against Urinary Tract Infection by Using Protein Display on Lactobacillus
| العنوان: | A New Approach for Designing a Potentially Vaccine Candidate against Urinary Tract Infection by Using Protein Display on Lactobacillus |
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| المؤلفون: | Gholamreza Goudarzi, Jalil Fallah-Mehrabadi, Fatemeh Farahani-Hatamabadi, Hedroosha Molla-Agha-Mirzaei, Sarah Najafi |
| المصدر: | Journal of Medical Bacteriology, Vol 1, Iss 3-4 (2015) |
| بيانات النشر: | Tehran University of Medical Sciences, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Vaccines, Probiotics, lcsh:R, Uropathogenic Escherichia coli, lcsh:Medicine, Urinary Tract Infection |
| الوصف: | Background: The prevalence of Urinary Tract Infection (UTI) is really high in the world. Escherichia coli is a major agent of UTI. One of the strategies for decreasing UTI infections is vaccine development. As the attachment is a really important stage in colonization and infection, at- tachment inhibition has an applied strategy. FimH protein is a major factor during bacterial colonization in urinary tract and could be used as a vaccine. Thus, it was considered in this research as a candidate anti- gen. Methods: The sequences of fimH and acmA genes were used for de- signing a synthetic gene. It was cloned to pET23a expression vector and transformed to E. coli (DE3) Origami. To confirm the expression of recombinant protein, SDS-PAGE and western blotting methods were used. Subsequently, recombinant protein was purified. On the other hand, Lactobacillus reuteri was cultured and mixed with FimH / AcmA recombinant protein. The rate of protein localization on lactobacillus surface was assessed using ELISA method. Results: It was showed that the recombinant protein was expressed in E. coli (DE3) Origami and purified by affinity chromatography. More- over, this protein could be localized on lactobacillus surface by 5 days. Conclusion: In current study, a fusion recombinant protein was pre- pared and displayed on L. reuteri surface. This strain could be used for animal experiment as a competitor against Uropathogenic E. coli (UPEC). Using manipulated probiotics strains instead of antibiotic ther- apy could decrease the antibiotic consumption and reduce multi-drug resistant strains. |
| اللغة: | English |
| تدمد: | 2322-2581 2251-8649 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doajarticles::b21df6af1d3f5c4ecaadf4db177f6cb5 https://jmb.tums.ac.ir/index.php/jmb/article/view/18 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doajarticles..b21df6af1d3f5c4ecaadf4db177f6cb5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23222581 22518649 |
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