In vitro Selection of Single-Stranded DNA Aptamers that Bind Human Pro-Urokinase
| العنوان: | In vitro Selection of Single-Stranded DNA Aptamers that Bind Human Pro-Urokinase |
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| المؤلفون: | Robert Sh. Beabealashvilli, Savochkina Lp, N. A. Skrypina |
| المصدر: | ChemInform. 36 |
| بيانات النشر: | Wiley, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Urokinase, Plasmin, Aptamer, General Medicine, In vitro, chemistry.chemical_compound, chemistry, Biochemistry, Nucleic acid, medicine, Plasminogen activator, DNA, Systematic evolution of ligands by exponential enrichment, medicine.drug |
| الوصف: | Single-chain pro-urokinase is an inactive proenzyme form of human urokinase (urinary plasminogen activator) with a Mr of 50,000 which is converted to the active two-chain form by catalytic amounts of plasmin. It is used for thrombolytic therapy of acute myocardial infarction and acute ischemic stroke. We have isolated single-stranded DNA molecules with significantly increased binding affinity for human pro-urokinase by SELEX (systematic evolution of ligands by exponential enrichment) procedure from a pool of 10(15) molecules containing 24 randomized positions which are flanked by defined regions. ssDNA from this library was hybridized with helper "fixture", thus allowing the central random chain to fold into complex three-dimentional shapes. Sequencing data from pro-urokinase aptamers obtained after 12 selection cycles displayed a highly conserved 12-14 base region. |
| تدمد: | 1522-2667 0931-7597 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::061000023f30e84ae5df461cd750eae7 https://doi.org/10.1002/chin.200505209 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........061000023f30e84ae5df461cd750eae7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15222667 09317597 |
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