Development and Validation of an Analytical Method for Simultaneous Estimation of Clotrimazole and its Impurity by RP-HPLC
| العنوان: | Development and Validation of an Analytical Method for Simultaneous Estimation of Clotrimazole and its Impurity by RP-HPLC |
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| المؤلفون: | Ketan V. Shah, Pankaj Kapupara, Priyanka M. Pandya |
| المصدر: | Research Journal of Pharmacy and Technology. 11:894 |
| بيانات النشر: | A and V Publications, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Chromatography, Materials science, Clotrimazole, High-performance liquid chromatography, Volumetric flow rate, chemistry.chemical_compound, chemistry, Impurity, Phase (matter), medicine, Pharmacology (medical), Methanol, Acetonitrile, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Retention time, medicine.drug |
| الوصف: | A simple, efficient and reproducible method for the simultaneous determination of Clotrimazole and its Impurity (2-Chlorphenyl diphenyl methanol) has been developed using reversed phase high performance liquid chromatography. The separation was carried out using a mobile phase consisting of methanol: acetonitrile (95:5 % v/v). Column used was Zorbax C18 (100 × 4.6 mm internal diameter) 3.5 µm with flow rate of 1 ml/min. The detection wavelength used was 225 nm. The retention time of Clotrimazole and 2-Chlorophenyl diphenyl methanol were 2.81 and 2.14 respectively. Linearity of Clotrimazole and its Impurity are in range of 10-80 µg/mL and 2-12 µg/mL respectively. Analytical parameters were calculated and a statistical evaluation included. |
| تدمد: | 0974-360X 0974-3618 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::0a7a06565c61786f286d7445a165bddf https://doi.org/10.5958/0974-360x.2018.00165.8 |
| رقم الأكسشن: | edsair.doi...........0a7a06565c61786f286d7445a165bddf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0974360X 09743618 |
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