Tomato (Solanum lycopersicum) variety discrimination and hybridization analysis based on the 5S rRNA region
| العنوان: | Tomato (Solanum lycopersicum) variety discrimination and hybridization analysis based on the 5S rRNA region |
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| المؤلفون: | Soon-Kwan Hong, Jin-Jun Xiang, Yan-Lin Sun, Young-Sik Kim, Jun-Pill Baek, Shi-Lin Zheng, Ho-Min Kang |
| المصدر: | Biotechnology & Biotechnological Equipment. 28:431-437 |
| بيانات النشر: | Informa UK Limited, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Genetic diversity, biology, Phylogenetic tree, Sequence analysis, fungi, food and beverages, biology.organism_classification, Variety (linguistics), Crop, 5S ribosomal RNA, Botany, Solanum, Phylogenetic relationship, Biotechnology |
| الوصف: | The tomato (Solanum lycopersicum) is a major vegetable crop worldwide. To satisfy popular demand, more than 500 tomato varieties have been bred. However, a clear variety identification has not been found. Thorough understanding of the phylogenetic relationship and hybridization information of tomato varieties is very important for further variety breeding. Thus, in this study, we collected 26 tomato varieties and attempted to distinguish them based on the 5S rRNA region, which is widely used in the determination of phylogenetic relations. Sequence analysis of the 5S rRNA region suggested that a large number of nucleotide variations exist among tomato varieties. These variable nucleotide sites were also informative regarding hybridization. Chromas sequencing of Yellow Mountain View and Seuwiteuking varieties indicated three and one variable nucleotide sites in the non-transcribed spacer (NTS) of the 5S rRNA region showing hybridization, respectively. Based on a phylogenetic tree constructed using the 5S rRNA sequences, we observed that 16 tomato varieties were divided into three groups at 95% similarity. Rubiking and Sseommeoking, Lang Selection Procedure and Seuwiteuking, and Acorn Gold and Yellow Mountain View exhibited very high identity with their partners. This work will aid variety authentication and provides a basis for further tomato variety breeding. |
| تدمد: | 1314-3530 1310-2818 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::0b8b99e5909a2ec24dd7c4278a3be47a https://doi.org/10.1080/13102818.2014.928499 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........0b8b99e5909a2ec24dd7c4278a3be47a |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 13143530 13102818 |
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