Although there are many studies that analyse and describe the distribution patterns of diverse organisms in South America at different scales, Paraguay has been poorly assessed from a biogeographic point of view. Some of the available contributions on the biogeography of Paraguay are based on different taxonomic groups, such as mammals, birds, reptiles, and plants, describing relationships between species and their habitats by using indices of similarity and cluster analysis. The main objective of this contribution is to identify areas of endemism based on the distribution of the 87 amphibian species known from Paraguay, and to compare the results with the three schemes of ecoregion proposed for the country. Eight areas of endemism were identified at different size of grids/scales, congruent with Dry Chaco, Atlantic Forest, Cerrado, Grasslands of Mesopotamia, Ñeembucú, and the Great American Chaco ecoregions.
