Chemoproteomic Profiling of O-GlcNAcylation in Caenorhabditis elegans
| العنوان: | Chemoproteomic Profiling of O-GlcNAcylation in Caenorhabditis elegans |
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| المؤلفون: | Guangshuo Ou, Xing Chen, Jingyang Wang, Wei Qin, Zhongyun Xie, Chu Wang |
| المصدر: | Biochemistry. 59:3129-3134 |
| بيانات النشر: | American Chemical Society (ACS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | O glcnacylation, biology, Quantitative proteomics, Computational biology, biology.organism_classification, Biochemistry, Phenotype, Caenorhabditis elegans |
| الوصف: | Genetic studies have revealed essential functions of O-linked N-acetylglucosamine (O-GlcNAc) modification in Caenorhabditis elegans. However, large-scale identification of O-GlcNAcylated proteins and mapping the modification sites in C. elegans remain relatively unexplored. By using a chemoproteomic strategy, we herein report the identification of 108 high-confidence O-GlcNAcylated proteins and 64 modification sites in C. elegans. Furthermore, quantitative proteomics upon altering O-GlcNAcylation show that the abundance of a large number of proteins are affected by O-GlcNAc. These proteins are involved in regulating reproduction and lifespan, which may correlate with the previously observed phenotypes in genetic studies. The data set in this study reveals the O-GlcNAc modification landscape in C. elegans and provides a valuable resource for dissecting the biological function of O-GlcNAcylation. |
| تدمد: | 1520-4995 0006-2960 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::0c4131d2a562db7568c8c6b10045e706 https://doi.org/10.1021/acs.biochem.9b00622 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........0c4131d2a562db7568c8c6b10045e706 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15204995 00062960 |
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