Burkholderia pseudomalleikillsCaenorhabditis elegansthrough virulence mechanisms distinct from intestinal lumen colonization
| العنوان: | Burkholderia pseudomalleikillsCaenorhabditis elegansthrough virulence mechanisms distinct from intestinal lumen colonization |
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| المؤلفون: | Soon-Keat Ooi, Song Hua Lee, Tian Yeh Lim, Sheila Nathan |
| المصدر: | Virulence. 3:485-496 |
| بيانات النشر: | Informa UK Limited, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Microbiology (medical), biology, Burkholderia pseudomallei, Pharyngeal pumping, Host–pathogen interaction, Immunology, Virulence, bacterial infections and mycoses, biology.organism_classification, medicine.disease_cause, Microbiology, Virology, Infectious Diseases, medicine, bacteria, Parasitology, Colonization, Escherichia coli, Bacteria, Caenorhabditis elegans |
| الوصف: | The nematode Caenorhabditis elegans is hypersusceptible to Burkholderia pseudomallei infection. However, the virulence mechanisms underlying rapid lethality of C. elegans upon B. pseudomallei infection remain poorly defined. To probe the host-pathogen interaction, we constructed GFP-tagged B. pseudomallei and followed bacterial accumulation within the C. elegans intestinal lumen. Contrary to slow-killing by most bacterial pathogens, B. pseudomallei caused fairly limited intestinal lumen colonization throughout the period of observation. Using grinder-defective mutant worms that allow the entry of intact bacteria also did not result in full intestinal lumen colonization. In addition, we observed a significant decline in C. elegans defecation and pharyngeal pumping rates upon B. pseudomallei infection. The decline in defecation rates ruled out the contribution of defecation to the limited B. pseudomallei colonization. We also demonstrated that the limited intestinal lumen colonization was not attributed to slowed host feeding as bacterial loads did not change significantly when feeding was stimulated by exogenous serotonin. Both these observations confirm that B. pseudomallei is a poor colonizer of the C. elegans intestine. To explore the possibility of toxin-mediated killing, we examined the transcription of the C. elegans ABC transporter gene, pgp-5, upon B. pseudomallei infection of the ppgp-5::gfp reporter strain. Expression of pgp-5 was highly induced, notably in the pharynx and intestine, compared with Escherichia coli-fed worms, suggesting that the host actively thwarted the pathogenic assaults during infection. Collectively, our findings propose that B. pseudomallei specifically and continuously secretes toxins to overcome C. elegans immune responses. |
| تدمد: | 2150-5608 2150-5594 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::0c42b819c990efb9f7632bb2f2acebc7 https://doi.org/10.4161/viru.21808 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........0c42b819c990efb9f7632bb2f2acebc7 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 21505608 21505594 |
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