STEROID METABOLISM IN THE PERFUSED HUMAN PLACENTA
| العنوان: | STEROID METABOLISM IN THE PERFUSED HUMAN PLACENTA |
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| المؤلفون: | A. Wolf, Christian Lauritzen, K. Musch, Werner Speidel, Jürgen R. Strecker |
| المصدر: | Acta Endocrinologica. 87:181-191 |
| بيانات النشر: | Oxford University Press (OUP), 1978. |
| سنة النشر: | 1978 |
| مصطلحات موضوعية: | medicine.medical_specialty, Endocrinology, Chemistry, Endocrinology, Diabetes and Metabolism, Internal medicine, medicine, Steroid Metabolism, Human placenta, General Medicine |
| الوصف: | A new model for the perfusion of human term-placentas has been developed for studies on the placental biogenesis of C-18 and C-19 steroids. For viability criteria, the glucose- and oxygen-consumption, regional perfusion control by dye-infusions or scanning after injection of 99Tc-labelled macroparticles, and the histological qualification were chosen. The recycled perfusate was investigated for the steroids oestrone (Oe1), oestradiol-17β (Oe2), oestriol (Oe3), 4-androstene-3,17-dione (A), testosterone (T), and human placental lactogen (HPL) by radioimmunoassay in controls and perfusions with the foetal steroid precursor dehydroepiandrosterone sulphate (DHA-S). In control perfusions, steroid hormones were found in constant ratios (Oe1:Oe2:Oe3:T:A = 30:1.5:100:0.35:1). Following the administration of 10 mg DHA-S for testing the metabolic capacity of the organ, high concentrations of Oe1 (90–720 ng/ml = 250–3970 % as compared to 100% pre-injection values) were found, shortly preceded by a rapid increase of A (66–1000 ng/ml = 100–16 000 %). A typical surge of T (5.3–147 ng/ml = 265–4640 %) preceded the normally slower increment of Oe2 (22–220 ng/ml = 1570–4330 %). The concentrations of Oe3 and HPL remained nearly unchanged. From different steroid patterns after DHA-S-load, two distinct responses of term-placentas could be differentiated: Group I (n=12) showed high concentrations of Oe1 (3200 ± 940 %), a small increase of T (1020 ± 500%), as well as low and delayed values of Oe2 (1660 ± 450%). In Group II (n = 5), values were high for T (3160 ± 1020%) and Oe2 (3300 ± 1110%), whereas Oe1 was found in a lower range (508 ± 302%). In contrast to in vivo findings in maternal venous blood after DHS-S injection to the mother, oestrone was found in perfusions as the main oestrogen fraction from DHA-S. Thus, the analysis of such metabolic differences might be of help in the interpretation of complex results from the DHA-S-loading test. |
| تدمد: | 1479-683X 0804-4643 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::0eafad26f77f26521d6b5aa4063cc056 https://doi.org/10.1530/acta.0.0870181 |
| رقم الأكسشن: | edsair.doi...........0eafad26f77f26521d6b5aa4063cc056 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1479683X 08044643 |
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