التفاصيل البيبلوغرافية
| العنوان: |
13C-TmDOTA as versatile thermometer compound for solid-state NMR of hydrated lipid bilayer membranes |
| المؤلفون: |
Matsumori Nobuaki, Michio Murata, Yuichi Umegawa, Yuya Tanaka |
| المصدر: |
Magnetic Resonance in Chemistry. 54:227-233 |
| بيانات النشر: |
Wiley, 2015. |
| سنة النشر: |
2015 |
| مصطلحات موضوعية: |
Chemistry, Resolution (electron density), Analytical chemistry, General Chemistry, 010402 general chemistry, 01 natural sciences, Temperature measurement, 030218 nuclear medicine & medical imaging, 0104 chemical sciences, NMR spectra database, 03 medical and health sciences, 0302 clinical medicine, Membrane, Solid-state nuclear magnetic resonance, Thermometer, Magic angle spinning, General Materials Science, Lipid bilayer |
| الوصف: |
Recent advances in solid-state nuclear magnetic resonance (NMR) techniques, such as magic angle spinning and high-power decoupling, have dramatically increased the sensitivity and resolution of NMR. However, these NMR techniques generate extra heat, causing a temperature difference between the sample in the rotor and the variable temperature gas. This extra heating is a particularly crucial problem for hydrated lipid membrane samples. Thus, to develop an NMR thermometer that is suitable for hydrated lipid samples, thulium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (TmDOTA) was synthesized and labeled with (13) C (i.e., (13) C-TmDOTA) to increase the NMR sensitivity. The complex was mixed with a hydrated lipid membrane, and the system was subjected to solid-state NMR and differential scanning calorimetric analyses. The physical properties of the lipid bilayer and the quality of the NMR spectra of the membrane were negligibly affected by the presence of (13) C-TmDOTA, and the (13) C chemical shift of the complex exhibited a large-temperature dependence. The results demonstrated that (13) C-TmDOTA could be successfully used as a thermometer to accurately monitor temperature changes induced by (1) H decoupling pulses and/or by magic angle spinning and the temperature distribution of the sample inside the rotor. Thus, (13) C-TmDOTA was shown to be a versatile thermometer for hydrated lipid assemblies. Copyright © 2015 John Wiley & Sons, Ltd. |
| تدمد: |
0749-1581 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::101fafd92506a6114ee4a1bfdcce5534
https://doi.org/10.1002/mrc.4371 |
| حقوق: |
CLOSED |
| رقم الأكسشن: |
edsair.doi...........101fafd92506a6114ee4a1bfdcce5534 |
| قاعدة البيانات: |
OpenAIRE |
