التفاصيل البيبلوغرافية
| العنوان: |
In vitroconfocal Raman micro-spectroscopic approach to screen the mode of action of Chinese herbs on oxidative damaged human sperm |
| المؤلفون: |
Hua-Lun Liang, Li-Juan Lv, Di-Ling Chen, Xin Yang, Jun Zhao |
| المصدر: |
Journal of Raman Spectroscopy. 47:749-756 |
| بيانات النشر: |
Wiley, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
0301 basic medicine, Active ingredient, 030219 obstetrics & reproductive medicine, Chemistry, Confocal, Oxidative phosphorylation, Chinese herbs, complex mixtures, Sperm, In vitro, Toxicology, 03 medical and health sciences, symbols.namesake, 030104 developmental biology, 0302 clinical medicine, Biochemistry, symbols, General Materials Science, Statistical analysis, Raman spectroscopy, Spectroscopy |
| الوصف: |
This paper presented a rapid and reliable approach to screen the antioxidant active ingredients on human sperm from Chinese herbs by means of confocal Raman micro-spectroscopy. For the first time with the proposed approach, we performed the studies of rationalizing the active ingredients from several Chinese herbs. In this study, the FeSO4/H2O2-induced oxidative damaged sperm after co-culture with compounds or active fraction extracted from some Chinese herbs commonly used in the treatment of male infertility were observed by using confocal Raman micro-spectroscopy. The Raman spectral fingerprints of the damaged sperm were very different from the normal ones, and the further statistical analysis were successfully used to support the results, these differences were shorten after co-culture with the compounds or active fraction we had chosen, and the results were almost consistent with the traditional clinical trial results and other published data, all which showed that these compounds or active fraction were the chemogenomics of the Chinese herbs on anti-oxidative damaged sperm. In brief, we demonstrated that by means of confocal Raman micro-spectroscopy, in vitro is a rapid, reliable and large-scale approach to screen the antioxidant active ingredients on human sperm from Chinese herbs. Copyright © 2016 John Wiley & Sons, Ltd. |
| تدمد: |
0377-0486 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::13b3aa1651067d942d6ae6e1763351c1
https://doi.org/10.1002/jrs.4894 |
| حقوق: |
CLOSED |
| رقم الأكسشن: |
edsair.doi...........13b3aa1651067d942d6ae6e1763351c1 |
| قاعدة البيانات: |
OpenAIRE |
