التفاصيل البيبلوغرافية
العنوان: |
Data from Cancer-Associated CD43 Glycoforms as Target of Immunotherapy |
المؤلفون: |
Giuseppe Scala, Ileana Quinto, Massimo Mallardo, Iris Scala, Franco Maria Buonaguro, Patrizia Bonelli, David Venzon, Fabrizio Dal Piaz, Antonio Barbieri, Claudio Arra, Francesco Paolo D'Armiento, Angela Santoni, Cristina Capuano, Ricciarda Galandrini, Enrico Iaccino, Cristina Falcone, Marco Schiavone, Annamaria de Laurentiis, Giuseppe Fiume, Camillo Palmieri, Franca Maria Tuccillo |
بيانات النشر: |
American Association for Cancer Research (AACR), 2023. |
سنة النشر: |
2023 |
الوصف: |
CD43 is a sialoglycosylated membrane protein that is involved in cell proliferation and differentiation. CD43 glycoforms that are recognized by the UN1 monoclonal antibody (mAb) were expressed in lymphoblastoid T-cell lines and solid tumors, such as breast, colon, gastric, and squamous cell lung carcinomas, while unexpressed in the normal counterparts. The cancer association of UN1/CD43 epitope suggested the possibility to use the UN1 mAb for tumor diagnosis and therapy. In this study, we show that the UN1 mAb was endowed with antitumor activity in vivo because its passive transfer inhibited the growth of UN1-positive HPB-ALL lymphoblastoid T cells in mice. Furthermore, we demonstrate that tumor inhibition was due to UN1 mAb-dependent natural killer–mediated cytotoxicity. By screening a phage-displayed random peptide library, we identified the phagotope 2/165 as a mimotope of the UN1 antigen, as it harbored a peptide sequence that was specifically recognized by the UN1 mAb and inhibited the binding of the UN1 mAb to UN1-positive tumor cells. On the basis of sequence homology with the extracellular region of CD43 (amino acids 64 to 83), the 2/165 peptide sequence was likely mimicking the protein core of the UN1/CD43 epitope. When used as vaccine in mice, the 2/165 phagotope raised antibodies against the UN1/CD43 antigen, indicating that the 2/165 phagotope mimicked the UN1 antigen structure, and could represent a novel immunogen for cancer immunotherapy. These findings support the feasibility of using monoclonal antibodies to identify cancer-associated mimotopes for immunotherapy. Mol Cancer Ther; 13(3); 752–62. ©2013 AACR. |
URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::15fb7320af0539a139896a014f755e2f https://doi.org/10.1158/1535-7163.c.6536283 |
حقوق: |
OPEN |
رقم الأكسشن: |
edsair.doi...........15fb7320af0539a139896a014f755e2f |
قاعدة البيانات: |
OpenAIRE |