Objective: The present study investigated the possible changes in respiratory function and mechanics in paralympic cyclists in comparison with cyclists. Design: Lung function (spirometry) and mechanics (impulse oscillometry) pre- and post-bronchodilator, the levels of FeNO and the peripheral and respiratory muscle strength were evaluated in paralympic cyclists [spinal injury from C5 (incomplete injury) until T11 (complete injury); (n=8)] and cyclists (n=12). Results: Paralympic cyclists presented reduced FVC, FEV1, PEF, VC In and MEF75%. Of note, only athletes presented response to bronchodilator, as observed for FEV and for FVC/FEV1. Respiratory impedance (Z5Hz), total respiratory system resistance (R5Hz) was impaired in paralympic cyclists. No differences were found hand grip and respiratory muscle strength and for FeNO comparing paralympic cyclists with cyclists. Conclusion: We conclude that para-cyclists present impaired lung function and mechanics and that such impairments are not correlated with reduction in the respiratory or peripheral muscle strength or with pulmonary inflammation.