Characterization of mutants deficient in N-terminal phosphorylation of the chloroplast ATP synthase subunit β
| العنوان: | Characterization of mutants deficient in N-terminal phosphorylation of the chloroplast ATP synthase subunit β |
|---|---|
| المؤلفون: | Deserah D Strand, Daniel Karcher, Stephanie Ruf, Anne Schadach, Mark A Schöttler, Omar Sandoval-Ibañez, David Hall, David M Kramer, Ralph Bock |
| المصدر: | Plant Physiology. 191:1818-1835 |
| بيانات النشر: | Oxford University Press (OUP), 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Physiology, Genetics, Plant Science |
| الوصف: | Understanding the regulation of photosynthetic light harvesting and electron transfer is of great importance to efforts to improve the ability of the electron transport chain to supply downstream metabolism. A central regulator of the electron transport chain is ATP synthase, the molecular motor that harnesses the chemiosmotic potential generated from proton-coupled electron transport to synthesize ATP. ATP synthase is regulated both thermodynamically and post-translationally, with proposed phosphorylation sites on multiple subunits. In this study we focused on two N-terminal serines on the catalytic subunit β in tobacco (Nicotiana tabacum), previously proposed to be important for dark inactivation of the complex to avoid ATP hydrolysis at night. Here we show that there is no clear role for phosphorylation in the dark inactivation of ATP synthase. Instead, mutation of one of the two phosphorylated serine residues to aspartate to mimic constitutive phosphorylation strongly decreased ATP synthase abundance. We propose that the loss of N-terminal phosphorylation of ATPβ may be involved in proper ATP synthase accumulation during complex assembly. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::17a8911594c91f3b6e16bba58d0ce416 https://doi.org/10.1093/plphys/kiad013 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........17a8911594c91f3b6e16bba58d0ce416 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
|---|