Real-time polymerase chain reaction (PCR) cycle threshold and Clostridioides difficile infection outcomes
| العنوان: | Real-time polymerase chain reaction (PCR) cycle threshold and Clostridioides difficile infection outcomes |
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| المؤلفون: | Byungwoo Choi, Abhishek Deshpande, Thomas G. Fraser, Gary W. Procop, Ken Koon Wong, Sandra S. Richter, Aaron N. Dunn, Robert S. Butler, Carlos M. Isada |
| المصدر: | Infection Control & Hospital Epidemiology. 42:1228-1234 |
| بيانات النشر: | Cambridge University Press (CUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Microbiology (medical), 0303 health sciences, medicine.medical_specialty, 030306 microbiology, Epidemiology, business.industry, Mortality rate, Retrospective cohort study, Odds ratio, Logistic regression, Confidence interval, Odds, 03 medical and health sciences, 0302 clinical medicine, Infectious Diseases, Real-time polymerase chain reaction, Internal medicine, Medicine, 030212 general & internal medicine, business, Prospective cohort study |
| الوصف: | Objective:Clostridioides difficile infection (CDI) causes significant morbidity and mortality; however, the diagnosis of CDI remains controversial. The primary aim of our study was to evaluate the association of polymerase chain reaction (PCR) cycle threshold (Ct) values with CDI disease severity, recurrence, and mortality among adult patients with CDI.Design:Retrospective cohort study.Setting:Single tertiary-care hospital.Patients:Adult patients diagnosed with hospital-onset, healthcare facility–associated CDI from June 2014 to September 2015.Methods:We performed a retrospective chart review of included patients. Univariate and multivariable logistic regression methods were used to evaluate the association between Ct values and CDI severity, 8-week recurrence, and 30-day mortality.Results:Among 318 included patients, 51% were male and the mean age was 62 years; ~32% of the patients developed severe CDI and 11% developed severe–complicated CDI. The 30-day all-cause mortality rate was 11% and the 8-week recurrence rate was 9.5%. The overall mean Ct value was 32.9 (range, 23–40). Multivariable analyses showed that lower values of PCR Ct were associated with increased odds of 30-day morality (odds ratio [OR] 0.83; 95% confidence interval [CI], 0.72–0.96) but were not independently associated with CDI severity (OR, 0.99; 95% CI, 0.90–1.09) or recurrence (OR, 0.88; 95% CI, 0.77–1.00).Conclusions:Our findings suggest that PCR Ct values at the time of diagnosis may have a limited predictive value and utility in clinical decision making for inpatients with CDI. Larger, prospective studies across different patient populations are needed to confirm our findings. |
| تدمد: | 1559-6834 0899-823X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::17ce87d104f71e9964853241fe404aec https://doi.org/10.1017/ice.2020.1405 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........17ce87d104f71e9964853241fe404aec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15596834 0899823X |
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