Effectiveness of crystal violet stain for localization of mitotic activity in oral squamous cell carcinoma
العنوان: | Effectiveness of crystal violet stain for localization of mitotic activity in oral squamous cell carcinoma |
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المؤلفون: | Sadia Minhas, Syed Sajjad Sarwar, Aneequa Sajjad, Muhammad Qasim Raza, Ihtesham-ud-Din Qureshi, Syeda Zaira Sajjad |
المصدر: | Journal of Fatima Jinnah Medical University. 13:166-169 |
بيانات النشر: | Fatima Jinnah Medical University, 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | Pathology, medicine.medical_specialty, business.industry, Diagnostic aid, Stain, Mitotic Count, Staining, chemistry.chemical_compound, chemistry, medicine, Mitotic Figure, Basal cell, Crystal violet, business, Mitosis |
الوصف: | Background: Mitotic figure counting is simplest and oldest method for determining proliferative activity of cell. It is considered as one of the important diagnostic aid in cancer pathology. Though advanced methods to evaluate dysplastic features are more precise and definite but expense and time makes them less practicable for routine use. Therefore an effort was made to use economical as well as simple approach involving crystal violet stain (1%) to study the mitotic figures in oral squamous cell carcinoma. Materials and Methods: This descriptive research included samples, consisting of thirty three cases of the oral squamous cell carcinoma (OSCC). Representative sections were stained with H&E stain and 1% crystal violet stain respectively. The stained sections were viewed under optical microscope to count mitotic figures for evaluating the effectiveness of 1% crystal violet stain. Data obtained was statistically analyzed by using sample t-test. Results: There was noteworthy increase in the mean mitotic count among the crystal violet stained sections of OSCC in contrast to the OSCC sections stained with H&E (P = 0.00). Conclusion:1% Crystal violet stain can be considered as one of the optimum stains to observe the mitotic figure. Practice of staining with 1% crystal violet during routine histopathological procedures will be cost effective and may be used as a selective stain. |
تدمد: | 2616-6291 2616-6461 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::196c65709ac64f73a4ada87720915a6e https://doi.org/10.37018/jfjmu.671 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi...........196c65709ac64f73a4ada87720915a6e |
قاعدة البيانات: | OpenAIRE |
تدمد: | 26166291 26166461 |
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