RECK and TIMP-2 mediate inhibition of MMP-2 and MMP-9 by Annona muricata
| العنوان: | RECK and TIMP-2 mediate inhibition of MMP-2 and MMP-9 by Annona muricata |
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| المؤلفون: | S. Lakshmi, A Achuthan, Ashna Alias, Jyotsna Nambiar, Catherine Joseph, P Divya, Muralidharan Vanuopadath, G Drishya, Abishek Kumar, Chinchu Bose, Damu Sunil Kumar, Bipin G. Nair, Geetha B. Kumar, Sobha V. Nair, Ayesha Sherif, S Sudarslal, Sanu K. Shaji |
| المصدر: | Journal of Biosciences. 45 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, biology, Chemistry, Endogeny, General Medicine, Tissue inhibitor of metalloproteinase, Matrix metalloproteinase, biology.organism_classification, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Extracellular matrix, Cell culture, Apoptosis, Cancer research, HT1080, General Agricultural and Biological Sciences, Annona muricata, 010606 plant biology & botany |
| الوصف: | Up-regulation of MMP-2 and MMP-9 plays a significant role in promoting cancer progression by degrading the components of the extracellular matrix, thereby enhancing the migration of tumor cells. Although the antiproliferative and apoptotic effect of Annona muricata is well established, its effect on MMP-2 and MMP-9, a major target in several types of cancers, has not been studied. Powdered samples of various parts of A. muricata like fruit, stem, seed, and twig extracted using aqueous methanol showed significant dose-dependent inhibition of MMP-2 and MMP-9 in a highly metastatic fibrosarcoma cell line, HT1080. Additionally, these extracts also up-regulated the expression of several endogenous inhibitors of MMP-2 and MMP-9 like REversion-inducing Cysteine-rich protein with Kazal motifs (RECK) and Tissue Inhibitor of Metalloproteinase- 2 (TIMP-2). Furthermore, primary cells developed from tumor tissues obtained from patients not exposed to chemotherapy, also exhibited similar results. Remarkably, the inhibition of MMP-2 and MMP-9 observed was tumor specific, with the A. muricata fruit extract showing only 2% inhibition in cells obtained from normal tissues, when compared to 60% inhibition observed in cells obtained from tumor samples. The present study elucidates a novel mechanism by which A. muricata extracts selectively exhibit their anti-cancer activity in tumor cells by down-regulating MMP-2 and MMP-9 that are important biomarkers in cancer. |
| تدمد: | 0973-7138 0250-5991 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::1b1348fb04e0d95871074ac2650d58dd https://doi.org/10.1007/s12038-020-00056-z |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........1b1348fb04e0d95871074ac2650d58dd |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 09737138 02505991 |
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