Detection of five viruses infecting dormant bulbs by TaqMan-based real-time RT-PCR
| العنوان: | Detection of five viruses infecting dormant bulbs by TaqMan-based real-time RT-PCR |
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| المؤلفون: | B. D. Quinn, G. R. G. Clover, Jason Shiller, T. Wei, B. S. M. Lebas |
| المصدر: | Australasian Plant Pathology. 41:93-98 |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Cucumber mosaic virus, Arabis mosaic virus, Plant virus, Tobacco rattle virus, Nucleic acid, TaqMan, Plant Science, Biology, biology.organism_classification, Virology, Virus, Bulb |
| الوصف: | Dormant bulbs may be infected by a number of viruses which are of potential economic importance for the horticultural industry. Five important viruses in bulbs were successfully detected by TaqMan-based real-time one-step RT-PCR assays: Arabis mosaic virus (ArMV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Tobacco rattle virus (TRV) and Tulip virus X (TVX). Nucleic acid of appropriate quality and quantity for RT-PCR was extracted from nine bulb species using the Qiagen RNeasy plant mini kit. Depending on the assay, the sensitivity of the real-time RT-PCRs varied from being the same sensitivity to 1,000 times more sensitive than the respective conventional RT-PCRs. The reliability of the TVX real-time RT-PCR was further assessed against conventional RT-PCR using leaves and bulbs of 24 Tulipa × hybrida plants. TVX was detected in twice the number of leaf samples than bulb samples for both conventional and real-time RT-PCRs. |
| تدمد: | 1448-6032 0815-3191 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::1b227fb3e585677df145a05ad5b05323 https://doi.org/10.1007/s13313-011-0095-1 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........1b227fb3e585677df145a05ad5b05323 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14486032 08153191 |
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