In vitro detection of porphyrin-producing wound bacteria with real-time fluorescence imaging
| العنوان: | In vitro detection of porphyrin-producing wound bacteria with real-time fluorescence imaging |
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| المؤلفون: | William Little, Jessica Bourke, Laura M Jones, Monique Y. Rennie, Andrea J Lopez, Andre Gomez, Ralph S. DaCosta, Allie Clinton Smith, Klara C Keim, Herman Ng, Jeffrey Kirman, Danielle Dunham |
| المصدر: | Future Microbiology. 15:319-332 |
| بيانات النشر: | Future Medicine Ltd, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Fluorescence-lifetime imaging microscopy, biology, Biofilm, Pathogenic bacteria, medicine.disease_cause, biology.organism_classification, Microbiology, Fluorescence, Porphyrin, Yeast, 030207 dermatology & venereal diseases, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, chemistry, medicine, Heme, Bacteria |
| الوصف: | Aim: Fluorescence imaging can visualize polymicrobial populations in chronic and acute wounds based on porphyrin fluorescence. We investigated the fluorescent properties of specific wound pathogens and the fluorescence detected from bacteria in biofilm. Methods: Utilizing Remel Porphyrin Test Agar, 32 bacterial and four yeast species were examined for red fluorescence under 405 nm violet light illumination. Polymicrobial biofilms, supplemented with δ-aminolevulinic acid, were investigated similarly. Results: A total of 28/32 bacteria, 1/4 yeast species and polymicrobial biofilms produced red fluorescence, in agreement with their known porphyrin production abilities. Conclusion: These results identify common wound pathogens capable of producing porphyrin-specific fluorescence and support clinical observations using fluorescence imaging to detect pathogenic bacteria in chronic wounds. |
| تدمد: | 1746-0921 1746-0913 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::1c5afd5efa47d1dbfa2ccf2ed6d8e320 https://doi.org/10.2217/fmb-2019-0279 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........1c5afd5efa47d1dbfa2ccf2ed6d8e320 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 17460921 17460913 |
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