Genetic heterogeneity and predominance of blaCTX-M-15 in cefotaxime-resistant Enterobacteriaceae isolates colonizing hospitalized children in Tunisia
| العنوان: | Genetic heterogeneity and predominance of blaCTX-M-15 in cefotaxime-resistant Enterobacteriaceae isolates colonizing hospitalized children in Tunisia |
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| المؤلفون: | S. Harbaoui, S. Ferjani, M.S. Abbassi, M. Saidani, T. Gargueh, M. Ferjani, Y. Hammi, I. Boutiba-Ben Boubaker |
| المصدر: | Letters in Applied Microbiology. 75:1460-1474 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Applied Microbiology and Biotechnology |
| الوصف: | Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have emerged as important nosocomial pathogens. Community infections by these organisms have been also reported and were associated with previous intestinal colonization. We aimed to characterize cefotaxime-resistant Enterobacteriaceae (CTX-R-En) isolated from hospitalized children in a Tunisian paediatric ward. Seventy CTX-R-En isolates were collected from 227 rectal swabs from hospitalized children in a paediatric ward. Antimicrobial susceptibility testing was determined according to the EUCAST guidelines. Isolates were characterized by polymerase chain reaction (PCR, genes encoding: ESBLs, pAmpC, carbapenemases, plasmid-mediated quinolone resistance, virulence factors in Escherichia coli and Klebsiella pneumoniae isolates, occurrence of classes 1 and 2 integrons, phylogenetic groups of E. coli isolates, ERIC-PCR and PCR-based replicon typing) and conjugal transfer experiments. In total, 65 out of 227 (28·6%) hospitalized children were colonized with CTX-M-R-En, and 70 isolates were identified. Isolates were 59 ESBL-, 7 plasmidic-AmpC (pAmpC)-, 3 ESBL+pAmpC-, and one ESBL+carbapenemase producers. The following bla genes were identified: blaCTX-M-15 (n = 54), blaCTX-M-1 (n = 5), blaCTX-M-9 (n = 2), blaCTX-M-13 (n = 1) and blaCTX-M-14 (n = 1), blaCMY-2 (n = 5), blaCMY-4 (n = 4), blaACC-1 (n = 1) and blaOXA-48 (n = 1). Our results showed that hospitalized children were colonized with various CTX-R-En-producing several beta-lactamase enzymes. |
| تدمد: | 1472-765X 0266-8254 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::1eaf37fbf3fec8249f311515d114d603 https://doi.org/10.1111/lam.13812 |
| حقوق: | EMBARGO |
| رقم الأكسشن: | edsair.doi...........1eaf37fbf3fec8249f311515d114d603 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1472765X 02668254 |
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