Reinvestigating the Coughing Rat Model of Pertussis To Understand Bordetella pertussis Pathogenesis
| العنوان: | Reinvestigating the Coughing Rat Model of Pertussis To Understand Bordetella pertussis Pathogenesis |
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| المؤلفون: | Sophia M. Kenney, Alexander M. Horspool, Claire O. Kelly, M. Allison Wolf, Caleb A. Kisamore, Megan A. DeJong, Dylan T. Boehm, Emel Sen-Kilic, Ting Y. Wong, Mariette Barbier, Graham J. Bitzer, Justin R. Bevere, F. Heath Damron, Jason Kang, Jesse M. Hall |
| المصدر: | Infection and Immunity. 89 |
| بيانات النشر: | American Society for Microbiology, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Bordetella pertussis, Lung, biology, Immunology, medicine.disease, biology.organism_classification, Vaccine efficacy, Microbiology, Neutrophilia, respiratory tract diseases, Cellular infiltration, Pathogenesis, Infectious Diseases, medicine.anatomical_structure, medicine, Parasitology, Nasal administration, medicine.symptom, Whooping cough |
| الوصف: | Bordetella pertussis is a highly contagious bacterium that is the causative agent of whooping cough (pertussis). Currently, acellular pertussis vaccines (aP, DTaP, and Tdap) are used to prevent pertussis disease. However, it is clear that the aP vaccine efficacy quickly wanes, resulting in the reemergence of pertussis. Furthermore, recent work performed by the CDC suggest that current circulating strains are genetically distinct from strains of the past. The emergence of genetically diverging strains, combined with waning aP vaccine efficacy, calls for reevaluation of current animal models of pertussis. In this study, we used the rat model of pertussis to compare two genetically divergent strains Tohama 1 and D420. We intranasally challenged 7-week-old Sprague-Dawley rats with 108 viable Tohama 1 and D420 and measured the hallmark signs/symptoms of B. pertussis infection such as neutrophilia, pulmonary inflammation, and paroxysmal cough using whole-body plethysmography. Onset of cough occurred between 2 and 4 days after B. pertussis challenge, averaging five coughs per 15 min, with peak coughing occurring at day 8 postinfection, averaging upward of 13 coughs per 15 min. However, we observed an increase of coughs in rats infected with clinical isolate D420 through 12 days postchallenge. The rats exhibited increased bronchial restriction following B. pertussis infection. Histology of the lung and flow cytometry confirm both cellular infiltration and pulmonary inflammation. D420 infection induced higher production of anti-B. pertussis IgM antibodies compared to Tohama 1 infection. The coughing rat model provides a way of characterizing disease manifestation differences between B. pertussis strains. |
| تدمد: | 1098-5522 0019-9567 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::1f1200bd29c316526dace021ec184203 https://doi.org/10.1128/iai.00304-21 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........1f1200bd29c316526dace021ec184203 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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