With the gaining attention on tissue engineering application especially on scaffold, various researchers are stimulating their study on mechanical, thermal, and morphological properties of a 3D porous. The scaffold itself can create biological alternative to repair or replace damage organs or tissues. Synthetic biopolymer-based scaffold are used most often in tissue engineering because of their adaptable mechanical properties and ease of processing. The most typical material used in scaffold fabrication are poly(lactic) acid (PLA). Thus, FDM is suggested as extrusion technique to fabricate the PLA based scaffold due to its high flexibility in material handling. Apart from that, the effect and contribution FDM parameter towards degradation rate and water absorption is evaluated. Thus, it is necessary to setup the process parameter including extrude temperature, speed, and layer thickness to obtain the optimization the scaffold structure in order to get appropriate results in degradation. Therefore, in this study, the goal is to investigate the effect of printing parameter on bioactivity of PLA scaffold by preparing simulated body fluid (SBF) and used it for scaffold immersion for 3, 7 and 14 days. Surface morphology is obtained by using SEM while ANOVA is used to determine the most contribute parameters towards bioactivity. As degradation occurred, the rate of degradation and water absorption are increased over time. Besides, ANOVA analysis shows that there are influence between the three parameters.
