Quantitative difference in bacterial abundance determined with each protocol for SYBR Green I and 4'6-diamidino-2-phenylindole (DAPI) methods
| العنوان: | Quantitative difference in bacterial abundance determined with each protocol for SYBR Green I and 4'6-diamidino-2-phenylindole (DAPI) methods |
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| المؤلفون: | Seiko Hara, Tomohiko Kikuchi, Tatuki Toda, Aki Imai, Satoru Taguchi, Akira Shibata |
| المصدر: | Plankton and Benthos Research. 2:63-66 |
| بيانات النشر: | The Plankton Society of Japan/The Japanese Association of Benthology, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Chromatography, Ecology, animal diseases, Aquatic Science, Biology, Oceanography, Molecular biology, eye diseases, 4 6 diamidino 2 phenylindole, chemistry.chemical_compound, chemistry, SYBR Green I, DAPI, Ecology, Evolution, Behavior and Systematics |
| الوصف: | Bacterial abundance determined with SYBR Green I and 4� 6-diamidino-2-phenylindole (DAPI) methods were compared using the protocol for each method employing epifluorescence microscopy. This comparison, using seawater samples that were col- lected from Sagami Bay, revealed that the abundance determined with the SYBR Green I method was on average 1.6 fold greater than that with the DAPI method. A model 2 regression analysis showed the following relationship: Log SYBR Green I�� 0.238� 1.08 Log DAPI (r 2 � 0.85, n� 68, p� 0.0001). These results suggest that when the method is switched between the SYBR Green I and DAPI methods, the difference in bacterial abundance determined by the two methods should be con- sidered for a quantitatively consistent assessment of the bacterial biomass in the ocean. |
| تدمد: | 1882-627X 1880-8247 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::212089e73151c89a109e5dd3faf10232 https://doi.org/10.3800/pbr.2.63 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........212089e73151c89a109e5dd3faf10232 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1882627X 18808247 |
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