Detection of tobacco rattle virus in nematodes by reverse transcription and polymerase chain reaction
| العنوان: | Detection of tobacco rattle virus in nematodes by reverse transcription and polymerase chain reaction |
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| المؤلفون: | Marja Korsman, Frank van der Wilk, F.C. Zoon |
| المصدر: | European Journal of Plant Pathology. 100:109-122 |
| بيانات النشر: | Springer Science and Business Media LLC, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | biology, Plant Science, Horticulture, biology.organism_classification, Virology, Molecular biology, Reverse transcriptase, Virus, law.invention, law, Tobacco rattle virus, Complementary DNA, Agarose gel electrophoresis, Nucleic acid, Tobravirus, Agronomy and Crop Science, Polymerase chain reaction |
| الوصف: | An assay, based on amplification of cDNA synthesized from genomic viral RNA, has been developed to detect tobacco rattle virus in infected plant material and viruliferous nematodes. A range of different TRV strains could be detected using the procedure developed. The presence of one to three viruliferous nematodes in a nematode suspension was sufficient for the detection of TRV. The minimum amount of purified virus detectable in the assay was 15 fg, indicating an increased sensitivity of the PCR-based assay as compared to serological detection methods, like ELISA. A dot-blot hybridization procedure was developed for the detection of the PCR products, making agarose gel electrophoresis dispensable. |
| تدمد: | 1573-8469 0929-1873 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::22851ec11776fe17ba5e6624ddf4363f https://doi.org/10.1007/bf01876245 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........22851ec11776fe17ba5e6624ddf4363f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15738469 09291873 |
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