Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells
| العنوان: | Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells |
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| المؤلفون: | Dorina Strieth, Alexander Mehring, Jonas Chodorski, Judith Stiefelmaier, Roland Ulber, Janik Haffelder |
| المصدر: | Plant Cell, Tissue and Organ Culture (PCTOC). 143:573-581 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0303 health sciences, food.ingredient, Methyl jasmonate, biology, Basilicum, Plant physiology, Horticulture, Meristem, Ocimum, biology.organism_classification, 01 natural sciences, Elicitor, 03 medical and health sciences, chemistry.chemical_compound, food, chemistry, Ursolic acid, Oleanolic acid, 030304 developmental biology, 010606 plant biology & botany |
| الوصف: | The application of plant suspension culture to produce valuable compounds, such as the triterpenoids oleanolic acid and ursolic acid, is a well-established alternative to the cultivation of whole plants. Cambial meristematic cells (CMCs) are a growing field of research, often showing superior cultivation properties compared to their dedifferentiated cell (DDC) counterparts. In this work, the first-time establishment of O. basilicum CMCs is demonstrated. DDCs and CMCs were cultivated in shake flasks and wave-mixed disposable bioreactors (wDBRs) and evaluated regarding triterpenoid productivity and biomass accumulation. CMCs showed characteristic small vacuoles and were found to be significantly smaller than DDCs. Productivities of oleanolic and ursolic acid of CMCs were determined at 3.02 ± 0.76 mg/(l*d) and 4.79 ± 0.48 mg/(l*d) after 19 days wDBR cultivation, respectively. These values were consistently higher than any productivities determined for DDCs over the observed cultivation period of 37 days. Elicitation with methyl jasmonate of DDCs and CMCs in shake flasks resulted in increased product contents up to 48 h after elicitor addition, with the highest increase found in CMCs at 232.30 ± 19.33% (oleanolic acid) and 192.44 ± 18.23% (ursolic acid) after 48 h. For the first time, cambial meristematic cells of Ocimum basilicum were established and cultivated in a disposable bioreactor system. These cells outperform dedifferentiated cells of the same organism regarding productivity. |
| تدمد: | 1573-5044 0167-6857 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2491eff76ddf62b4cfc4fdd195cbfb4f https://doi.org/10.1007/s11240-020-01942-y |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........2491eff76ddf62b4cfc4fdd195cbfb4f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15735044 01676857 |
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