Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics
| العنوان: | Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics |
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| المؤلفون: | Nora Cesbron, Alexandre Sydor, B. Le Bizec, G. Dervilly-Pinel, Yann Guitton, Anne-Lise Royer |
| المصدر: | Metabolomics. 13 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Chromatography, Serial dilution, Endocrinology, Diabetes and Metabolism, 010401 analytical chemistry, Clinical Biochemistry, Extraction (chemistry), Context (language use), Biology, 01 natural sciences, Biochemistry, 0104 chemical sciences, 03 medical and health sciences, 030104 developmental biology, Metabolomics, Metabolome, Sample preparation, Animal species, Feces |
| الوصف: | Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites. In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting. The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces. A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed. The workflow generated repeatable and informative fingerprints for robust metabolome characterization. |
| تدمد: | 1573-3890 1573-3882 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2585f1467184d55d72c7caac180f2e85 https://doi.org/10.1007/s11306-017-1233-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........2585f1467184d55d72c7caac180f2e85 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15733890 15733882 |
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