Identification of allergic proteins of Flower tail shrimp (Metapenaeus dobsonii)
| العنوان: | Identification of allergic proteins of Flower tail shrimp (Metapenaeus dobsonii) |
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| المؤلفون: | T V Sankar, Satyen Kumar Panda, S.J. Laly |
| المصدر: | Journal of Food Science and Technology. 56:5415-5421 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0303 health sciences, Allergy, animal structures, biology, fungi, Metapenaeus, food and beverages, biology.organism_classification, medicine.disease_cause, medicine.disease, Immunoglobulin E, Tropomyosin, Crustacean, Shrimp, 03 medical and health sciences, 0302 clinical medicine, Allergen, 030228 respiratory system, medicine, biology.protein, Food science, Shellfish, 030304 developmental biology, Food Science |
| الوصف: | Allergy to seafood mainly fish and shellfish has been reported. Tropomyosin is recognized as a major allergen in many crustaceans especially shrimps. A study was carried out to identify the IgE reactive proteins of allergic nature in Flower tail shrimp (Metapenaeus dobsonii), a frequently consumed shrimp in India mainly in dried form. Protein profiling and identification of IgE reactive proteins in raw and cooked extracts of Flower tail shrimp was carried out by SDS-PAGE and immunoblotting using sera of 13 patients having allergic reactions on consumption of shrimp and positive to skin prick test. The IgE binding ability was determined by ELISA and it was found higher in the case of cooked extracts ranging from 0.244 to 0.440 at 490 nm. The SDS-PAGE of raw extract revealed many protein bands between 205 and 6.5 KDa, while in the case of cooked extracts bands of 36, 20, 29 and 70 KDa were prominent. Heat resistant protein of 37 KDa, tropomyosin was showing a clear immune reaction both in the case of raw and cooked extracts was identified as the major allergen by all the patient sera. The minor proteins of raw extract identified by immune reaction are 50, 75 and 100 KDa. The identified allergen can be used for the diagnosis and management of shrimp allergy. |
| تدمد: | 0975-8402 0022-1155 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::26c7d5e85a0920488216b1345dbfefc0 https://doi.org/10.1007/s13197-019-04012-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........26c7d5e85a0920488216b1345dbfefc0 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 09758402 00221155 |
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