التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of Two Enzyme Immunoassays for the Detection of the Cocaine Metabolite Benzoylecgonine in 1,398 Urine Specimens |
| المؤلفون: |
Alphonse Poklis, Carl E. Wolf, Lisa Tarnai-Moak, Sarah Carney |
| المصدر: |
Journal of Clinical Laboratory Analysis. 26:130-135 |
| بيانات النشر: |
Wiley, 2012. |
| سنة النشر: |
2012 |
| مصطلحات موضوعية: |
Microbiology (medical), Chromatography, medicine.diagnostic_test, Metabolite, Biochemistry (medical), Clinical Biochemistry, False Negative Reactions, Public Health, Environmental and Occupational Health, Hematology, Assay sensitivity, Urine, Medical Laboratory Technology, chemistry.chemical_compound, chemistry, Immunoassay, medicine, Benzoylecgonine, Immunology and Allergy, Gas chromatography, Gas chromatography–mass spectrometry |
| الوصف: |
Background Benzoylecgonine (BE) is the primary urinary metabolite of cocaine. Two enzyme immunoassays were evaluated for the detection of BEin urine with a 300 ng/ml cutoff: the DRI® Cocaine Metabolite Assay and Lin-Zhi International's (LZ) Cocaine Metabolite Enzyme Immunoassay. Methods This study involved 1,398 urine specimens from criminal justice and pain management programs. Gas chromatography/mass spectrometry (GC/MS) data were obtained for presumptive positives, and for negative urine specimens yielding responses significantly above the negative control. Results Approximately 46% (644) of the specimens yielded positive results by DRI, and 47% (664) were positive by LZ. One specimen screened positive with both assays but was found to have a nondetectable BE concentration by GC/MS, indicating one false positive for each assay. Twenty-one specimens yielding negative DRIresults contained BEabove 300 ng/ml, and 29 specimens yielded false negatives with the LZassay. Therefore, the overall agreement between both immunoassays and GC/MSresults was 98%. Assay sensitivity was 0.968 (DRI) and 0.958 (LZ); the selectivity for both assays was 0.999. Urine specimens containing cocaine, additional cocaine metabolites, and other drugs were also tested. No cross-reactivity was observed. Conclusion Both the DRIand LZassays provide a precise, reliable method for the routine detection of BEin urine. J. Clin. Lab. Anal. 26:130-135, 2012. © 2012 Wiley Periodicals, Inc. |
| تدمد: |
0887-8013 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::28ed1bd4a56e7227a37b62193fe4c50d
https://doi.org/10.1002/jcla.21498 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.doi...........28ed1bd4a56e7227a37b62193fe4c50d |
| قاعدة البيانات: |
OpenAIRE |
