Cytokine phenotype of human autoreactive T cell clones specific for the immunodominant myelin basic protein peptide (83-99)
| العنوان: | Cytokine phenotype of human autoreactive T cell clones specific for the immunodominant myelin basic protein peptide (83-99) |
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| المؤلفون: | Marco Vergelli, Roland Martin, T. Huang, Bernhard Hemmer, P. A. Calabresi, Henry F. McFarland |
| المصدر: | Journal of Neuroscience Research. 45:852-862 |
| بيانات النشر: | Wiley, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | biology, medicine.medical_treatment, T cell, T-cell receptor, Myelin basic protein, Proinflammatory cytokine, Cellular and Molecular Neuroscience, Myelin, Cytokine, medicine.anatomical_structure, Immunology, biology.protein, medicine, Cytokine secretion, IL-2 receptor |
| الوصف: | Experimental allergic encephalomyelitis (EAE), an animal model resembling multiple sclerosis (MS), is mediated by myelin antigen-specific CD4+ T cells secreting cytokines such as interferon-gamma (IFN-gamma), tumor necrosis factor-beta (TNF-beta), and the proinflammatory cytokine TNF-alpha-all associated with the T-helper-1 (Th1) T cell subset. Based on numerous similarities between MS and EAE, it has been postulated that Th1-like T cells are involved in the pathogenesis of MS. Production of proinflammatory cytokines such as IFN-gamma and, in particular, TNF-alpha/beta by autoreactive T cells is considered crucial for the initiation and amplification of inflammatory brain lesions and possibly also for direct myelin damage. In contrast, regulatory cytokines such as interleukin-4 (IL-4), IL-10, and IL-13, which are associated with the Th2-like phenotype, may play a role in the resolution of relapses. Although the human T cell response to myelin basic protein (MBP) is well characterized in terms of antigen specificity, HLA restriction, and T cell-receptor (TCR) usage, little is known about the cytokine pattern of these autoreactive T cells. To gain such information, conditions for studying cytokine secretion by human autoreactive T cell clones (TCC) were established. The cytokine secretion profile of human autoreactive CD4+ TCC, specific for myelin basic protein peptide (83-89) [MBP(83-99)], a candidate autoantigen in MS, was investigated. Our results show that TCC cytokine production in long-term culture was stable. In addition, the correlation of various cytokines within specific TCC revealed differences compared to murine T cells. The comparison of 30 human MBP (83-99)-specific TCC demonstrated heterogeneity in cytokine secretion, with a continuum between Th1- and Th2-like cells rather than distinct Th1 or Th2 subsets. These data are important for further investigation of the potential role of cytokines in the inflammatory process of MS, and provide a powerful tool to investigate therapeutic interventions with respect to their influence on cytokine secretion of autoreactive T cells. |
| تدمد: | 1097-4547 0360-4012 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::29011e83d61d058ebc49a50ac2856fbb https://doi.org/10.1002/(sici)1097-4547(19960915)45:6<852::aid-jnr22>3.0.co;2-w |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........29011e83d61d058ebc49a50ac2856fbb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974547 03604012 |
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