Thermostable glucoamylase-type enzyme fromBacillus acidocaldarius RP1
| العنوان: | Thermostable glucoamylase-type enzyme fromBacillus acidocaldarius RP1 |
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| المؤلفون: | Yusdiana Ermayadhie, Yuyu Yuliani, Redha Putra, Muliawati Sindumarta, Dessy Natalia |
| المصدر: | Biochemistry and Molecular Biology Education. 30:398-400 |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Ammonium sulfate, Hot spring, Chromatography, Microorganism, Thermophile, Sonication, West java, Biology, Biochemistry, chemistry.chemical_compound, Bacillus acidocaldarius, Enzyme, chemistry, Molecular Biology |
| الوصف: | We present an experiment that enables student to understand the properties of thermostable glucoamylase-type enzyme produced by thermophilic Bacillus acidocaldarius RP1 isolated from Cimanggu Hot Spring, West Java, Indonesia. In addition, the students also gain experience working with a thermostable microorganism and some techniques frequently used in biochemistry, e.g. ultrasonication, ammonium sulfate fractionation, and spectrophotometric analysis. The glucoamylase-type enzyme activities were detected both as cell-associated and in the culture supernatant. The 20–40% saturated ammonium sulfate fraction of glucoamylase-type enzyme exhibited an optimum temperature of 65 °C and an optimum pH of 4.5. The enzyme showed a Vmax of 600 milliunits/mg and Km of 11.7 mg/ml. |
| تدمد: | 1539-3429 1470-8175 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2b357e28a88259cffe2b3a2db5caf3fb https://doi.org/10.1002/bmb.2002.494030060137 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........2b357e28a88259cffe2b3a2db5caf3fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15393429 14708175 |
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