IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells
| العنوان: | IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells |
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| المؤلفون: | B S Bochner, D A Klunk, S A Sterbinsky, R L Coffman, R P Schleimer |
| المصدر: | The Journal of Immunology. 154:799-803 |
| بيانات النشر: | The American Association of Immunologists, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Previous studies with human umbilical vein endothelial cells (HUVEC) have shown that the cytokine IL-4 induces adherence of human eosinophils, but not neutrophils, because of its ability to selectively induce surface expression of vascular cell adhesion molecule-1 (VCAM-1). Because the cytokine IL-13 shares a number of biologic properties with IL-4, we examined the effect of IL-13 on the expression and function of adhesion molecules on HUVEC. Incubation of HUVEC for 4 to 48 h with IL-13 (0.1 to 15 U/ml) induced surface expression of VCAM-1, as detected by indirect immunofluorescence and flow cytometry, without significantly affecting expression of E-selectin or intercellular adhesion molecule-1. The kinetics and maximal IL-13-induced expression of VCAM-1 were similar to those seen with IL-4. Treatment of HUVEC with an optimal concentration of IL-13 (15 U/ml for 24 h) induced adhesiveness for eosinophils, but not for neutrophils, and adhesion was completely inhibited by mAb recognizing VCAM-1 or alpha 4 integrin (CD49d). These results demonstrate that IL-13, like IL-4, selectively stimulates HUVEC to express functional cell surface VCAM-1 and suggest a possible role for IL-13 in promoting VCAM-1/alpha 4 integrin-dependent accumulation of eosinophils during allergic and other inflammatory reactions in vivo. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2ba45b48389051b9cdaa2aac1bcb25c1 https://doi.org/10.4049/jimmunol.154.2.799 |
| رقم الأكسشن: | edsair.doi...........2ba45b48389051b9cdaa2aac1bcb25c1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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