Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II
| العنوان: | Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II |
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| المؤلفون: | Cristina Battaglia, G. De Bellis, L. Rossi Bernardi, C. Consolandi, Giuliana Salani |
| المصدر: | BioTechniques. 29:78-81 |
| بيانات النشر: | Future Science Ltd, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | chemistry.chemical_compound, chemistry, Biochemistry, Non destructive, Fluorescent staining, DNA microarray, General Biochemistry, Genetics and Molecular Biology, DNA, Biotechnology, Staining |
| الوصف: | A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR® green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preliminary control, which may avoid further analytical steps that lead to the waste of precious biological samples, a fully reversible staining procedure is performed that produces an array ready for subsequent use. |
| تدمد: | 1940-9818 0736-6205 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2de1c5b1f9b01241a4cea214ea01da01 https://doi.org/10.2144/00291st01 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........2de1c5b1f9b01241a4cea214ea01da01 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 19409818 07366205 |
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