LabVIEW is an interactive program development and execution system in which one creates programs using a graphical notation. This paper gives an idea about development of a graphical user interface for digital collection, and how one can control an instrument with the use of LabVIEW. It proposed the use of LabVIEW for controlling an optical polarimeter, which is based on Spartan 3E FPGA system. Optical Polarimeter works on the principle of rapid modulation of light, and measures the angle of rotation caused by passing polarized light through an optically active substance. A GUI is developed in LabVIEW to communicate with optical polarimeter in accordance with user’s requirement. Serial connection takes place between FPGA board and host PC through LabVIEW. At a time, one data bit is send during serial communication and a complete byte is being sent with a start bit, stop bit, and parity bit. Entire system consists of two parts: Spartan 3E FPGA programming device and NI LabVIEW software.