The extrafollicular-to-follicular transition of human B lymphocytes: induction of functional globotriaosylceramide (CD77) on high threshold occupancy of CD40
| العنوان: | The extrafollicular-to-follicular transition of human B lymphocytes: induction of functional globotriaosylceramide (CD77) on high threshold occupancy of CD40 |
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| المؤلفون: | John Gordon, Lynne M. Roberts, Michelle J. Holder, Natalie McCloskey, John D. Pound, J. Michael Lord, Julie M. Williams |
| المصدر: | European Journal of Immunology. 29:3236-3244 |
| بيانات النشر: | Wiley, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | CD86, CD40, T cell, Immunology, Globotriaosylceramide, Germinal center, Priming (immunology), Cell cycle, Biology, CD38, Cell biology, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, biology.protein, medicine, Immunology and Allergy |
| الوصف: | Amongst lymphocytes, expression of CD77 (globotriaosylceramide, Gb3) is exclusive to B cells of the germinal center (GC). Its acquisition by extrafollicular B cells may thus herald their commitment to a follicular response. Here we show that high threshold occupancy of CD40 by its cognate ligand (CD40L) promotes rapid induction of CD77 expression in non-GC (CD38(lo)) B cells. The kinetics of CD77 acquisition mirrored those of GC-related markers CD95 and CD86 but contrasted with the more delayed increase in CD38 expression. Induction of CD77 was not a simple consequence of cell cycle entry: other conditions of stimulation equally capable of driving proliferation failed to promote CD77 expression. CD77 was functional in that cells were now sensitive to Verotoxin-1, an Escherichia coli-derived ligand of Gb3. These data indicate that acquisition by extrafollicular B cells of CD77 results from high threshold occupancy of CD40, a situation that should be reached physiologically only once a critical level of T cell priming has been achieved. |
| تدمد: | 1521-4141 0014-2980 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2fce426005dd7470f1cca4bac8a8c156 https://doi.org/10.1002/(sici)1521-4141(199910)29:10<3236::aid-immu3236>3.0.co;2-t |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........2fce426005dd7470f1cca4bac8a8c156 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15214141 00142980 |
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