Immunohistochemical study of BRAF V600E mutant protein expression in high-grade sarcomas
| العنوان: | Immunohistochemical study of BRAF V600E mutant protein expression in high-grade sarcomas |
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| المؤلفون: | Shengle Zhang, Kerry Whiting, Alfredo L. Valente, Jamie Tull, Charlene Maciak |
| المصدر: | Journal of Solid Tumors. 5 |
| بيانات النشر: | Sciedu Press, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Cancer Research, Pathology, medicine.medical_specialty, Mutation, business.industry, Melanoma, Thyroid, medicine.disease, medicine.disease_cause, Staining, Histiocytosis, medicine.anatomical_structure, Oncology, Mutant protein, Cancer research, medicine, Immunohistochemistry, Radiology, Nuclear Medicine and imaging, Vemurafenib, business, medicine.drug |
| الوصف: | BRAF V600E is a mutation present in numerous neoplasms, including melanomas, thyroid, colorectal and ovarian carcinomas, gastrointestinal stromal tumors, and Langerhans’ cell histiocytosis. Vemurafenib, a BRAF V600E kinase inhibitor has been successfully used in the treatment of melanoma. The role of this mutation in unclassified high-grade sarcomas, malignancies with very limited treatment options, has not been widely studied. Because of the availability of a highly sensitive and specific antibody (VE1) against the BRAF V600E mutant protein, we tested 48 cases of unclassified high-grade sarcomas. Cytoplasmic expression intensity was graded as negative (0), or positive (2+ or 3+) by two pathologists and a pathology resident. Forty one out of 48 specimens remained intact in the cores after immunohistochemistry (IHC) processing. Six of the 41 cases (15%) were scored as positive. In addition, non-specific nuclear staining was detected in 12/88 cores (14%). The 6 positive cases were tested for the BRAF V600E mutation by RT-PCR, and all were negative. Based on these results, we concluded that BRAF V600E mutation is rare in unclassified high-grade sarcomas, and because of the non-specific staining, results should be interpreted with caution. |
| تدمد: | 1925-4075 1925-4067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::30663814f23678fb9a58b1c662279715 https://doi.org/10.5430/jst.v5n1p44 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........30663814f23678fb9a58b1c662279715 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19254075 19254067 |
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