In order to explore the possibility of using repeatedly recycling waste concrete as aggregate to produce structural concrete, experimental research was carried out on the structural properties of three kinds of recycled fine aggregate (RFA) with two-regeneration cycles, two single and one mixed. The results showed that the quality of all RFAs meet the needs of Grade Ⅲ in GB/T25177-2010 and Level L in JISA 5023-2000. The degree of performance degradation is observed to enhance with increasing recycling cycle when taking the same preparation process of recycled aggregate.