Subnanosecond fluorescence spectroscopy of human serum albumin as a method to estimate the efficiency of the depression therapy
| العنوان: | Subnanosecond fluorescence spectroscopy of human serum albumin as a method to estimate the efficiency of the depression therapy |
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| المؤلفون: | E. Misionzhnik, T. I. Syrejshchikova, N. M. Maksimova, Yu. A. Gryzunov, M. G. Uzbekov, N. V. Smolina, A. A. Komar |
| المصدر: | Laser Physics. 20:1074-1078 |
| بيانات النشر: | Pleiades Publishing Ltd, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Chemistry, Fluorescence spectrometry, Albumin, Condensed Matter Physics, Ligand (biochemistry), Human serum albumin, Fluorescence, Industrial and Manufacturing Engineering, Atomic and Molecular Physics, and Optics, Fluorescence spectroscopy, Nuclear magnetic resonance, Blood serum, medicine, Spectroscopy, Instrumentation, medicine.drug |
| الوصف: | The efficiency of the therapy of psychiatric diseases is estimated using the fluorescence measurements of the conformational changes of human serum albumin in the course of medical treatment. The fluorescence decay curves of the CAPIDAN probe (N-carboxyphenylimide of the dimethylaminonaphthalic acid) in the blood serum are measured. The probe is specifically bound to the albumin drug binding sites and exhibits fluorescence as a reporter ligand. A variation in the conformation of the albumin molecule substantially affects the CAPIDAN fluorescence decay curve on the subnanosecond time scale. A subnanosecond pulsed laser or a Pico-Quant LED excitation source and a fast photon detector with a time resolution of about 50 ps are used for the kinetic measurements. The blood sera of ten patients suffering from depression and treated at the Institute of Psychiatry were preliminary clinically tested. Blood for analysis was taken from each patient prior to the treatment and on the third week of treatment. For ten patients, the analysis of the fluorescence decay curves of the probe in the blood serum using the three-exponential fitting shows that the difference between the amplitudes of the decay function corresponding to the long-lived (9 ns) fluorescence of the probe prior to and after the therapeutic procedure reliably differs from zero at a significance level of 1% (p < 0.01). |
| تدمد: | 1555-6611 1054-660X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::325e5b31e9ef6f93103a2edb4ebc37e5 https://doi.org/10.1134/s1054660x10100063 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........325e5b31e9ef6f93103a2edb4ebc37e5 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15556611 1054660X |
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