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Immobilization of proteins on magnetic nanoparticles

التفاصيل البيبلوغرافية
العنوان: Immobilization of proteins on magnetic nanoparticles
المؤلفون: Wen-Chien Lee, Tzu-Hsien Wang
المصدر: Biotechnology and Bioprocess Engineering. 8:263-267
بيانات النشر: Springer Science and Business Media LLC, 2003.
سنة النشر: 2003
مصطلحات موضوعية: chemistry.chemical_classification, biology, Biomedical Engineering, Nanoparticle, Bioengineering, Trypsin, Applied Microbiology and Biotechnology, Divalent, chemistry.chemical_compound, chemistry, Fluorescence microscope, biology.protein, medicine, Biophysics, Magnetic nanoparticles, Organic chemistry, DNA, Biotechnology, Avidin, medicine.drug, Carbodiimide
الوصف: Magnetic nanoparticles prepared from an alkaline solution of divalent and trivalent iron ions could covalently bind protein via the activation ofN-ethyl-N-(3-dimethylaminopropyl) carbodiimide (EDC). Trypsin and avidin were taken as the model proteins for the formation of protein-nanoparticle conjugates. The immobilized yield of protein increased with molar ratio of EDC/nanoparticle. Higher concentrations of added protein could yield higher immobilized protein densities on the particles. In contrast to EDC, the yields of protein immobilization via the activation of cyanamide were relatively lower. Nanoparticles bound with avidin could attach a single-stranded DNA through the avidin-biotin interaction and hybridize with a DNA probe. The DNA hybridization was confirmed by fluorescence microscopy observations. Immobilized DNA on nanoparticles by this technique may have widespread applicability to the detection of specific nucleic acid sequence and targeting of DNA to particular cells.
تدمد: 1976-3816
1226-8372
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::34b730ab3415ad9e0aaef993af0358af
https://doi.org/10.1007/bf02942276
حقوق: CLOSED
رقم الأكسشن: edsair.doi...........34b730ab3415ad9e0aaef993af0358af
قاعدة البيانات: OpenAIRE
الوصف
تدمد:19763816
12268372