Communication between Eσ54, promoter DNA and the conserved threonine residue in the GAFTGA motif of the PspF σ54-dependent activator during transcription activation
| العنوان: | Communication between Eσ54, promoter DNA and the conserved threonine residue in the GAFTGA motif of the PspF σ54-dependent activator during transcription activation |
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| المؤلفون: | Matthew Chaney, Siva R. Wigneshweraraj, Angel Ernesto Dago, Patricia Bordes, Martin Buck, Enrique Morett |
| المصدر: | Molecular Microbiology. 54:489-506 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Stereochemistry, Activator (genetics), Promoter, Biology, Microbiology, Molecular biology, Conserved sequence, chemistry.chemical_compound, Protein structure, chemistry, Transcription (biology), RNA polymerase, Phage shock, Molecular Biology, DNA |
| الوصف: | Conversion of Esigma(54) closed promoter complexes to open promoter complexes requires specialized activators which are members of the AAA (ATPases Associated with various cellular Activities) protein family. The ATP binding and hydrolysis activity of Esigma(54) activators is used in an energy coupling reaction to remodel the Esigma(54) closed promoter complex and to overcome the sigma(54)-imposed block on open complex formation. The remodelling target for the AAA activator within the Esigma(54) closed complex includes a complex interface contributed to by Region I of sigma(54), core RNA polymerase and a promoter DNA fork junction structure, comprising the Esigma(54) regulatory centre. One sigma(54) binding surface on Esigma(54) activators is a conserved sequence known as the GAFTGA motif. Here, we present a detailed characterization of the interaction between Region I of sigma(54) and the Escherichia coli AAA sigma(54) activator Phage shock protein F. Using Esigma(54) promoter complexes that mimic different conformations adopted by the DNA during open complex formation, we investigated the contribution of the conserved threonine residue in the GAFTGA motif to transcription activation. Our results suggest that the organization of the Esigma(54) regulatory centre, and in particular the conformation adopted by the sigma(54) Region I and the DNA fork junction structure during open complex formation, is communicated to the AAA activator via the conserved T residue of the GAFTGA motif. |
| تدمد: | 1365-2958 0950-382X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::35b36e9c7cd9c09da78b9c3f7d7cb1e9 https://doi.org/10.1111/j.1365-2958.2004.04280.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........35b36e9c7cd9c09da78b9c3f7d7cb1e9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652958 0950382X |
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