A continuously monitored colorimetric method for detection of Mycobacterium tuberculosis complex in sputum
| العنوان: | A continuously monitored colorimetric method for detection of Mycobacterium tuberculosis complex in sputum |
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| المؤلفون: | Marimoto S, Leonard Maboko, Guerra H, Froeschl G, Andrea Rachow, Mapamba D, Norbert Heinrich, Nyanda E. Ntinginya, Butler A, Kohlenberg A, Joseph J, Michael Hoelscher, Framhein A, Gabriel Rojas-Ponce, Ngatemelela D, Mlundi R, Chacha Mangu |
| المصدر: | The International Journal of Tuberculosis and Lung Disease. 17:1607-1612 |
| بيانات النشر: | International Union Against Tuberculosis and Lung Disease, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Pathology, medicine.medical_specialty, Bacilli, Tuberculosis, Time to detection, biology, Liquid culture, business.industry, medicine.disease, biology.organism_classification, Microbiology, Infectious Diseases, Treatment success, Mycobacterium tuberculosis complex, medicine, Sputum, medicine.symptom, business |
| الوصف: | SETTING Mbeya, Tanzania. OBJECTIVE To develop a new liquid culture method to detect Mycobacterium tuberculosis complex (MTC) in sputum using 2,3-diphenyl-5-thienyl-(2)-tetrazolium (STC), the nitrate reductase assay (NRA) and p-nitrobenzoic acid (PNB). DESIGN Ninety-three sputum samples collected from 18 tuberculosis patients were decontaminated with N-acetyl-L-cysteine-sodium hydroxide using MGIT™ 960 and in STC-NRA cultures, both in the presence and in the absence of PNB, an inhibitor of MTC growth. The reduction of STC by colour change indicated mycobacterial growth; NRA was then performed to confirm MTC. RESULTS STC-NRA culture was positive for acid-fast bacilli in 66/93 (71%) samples, of which 60/93 (64.5%) were identified as MTC-positive and 6/93 (6.5%) as indeterminate mycobacteria. MGIT indicated MTC in 59/93 (63.4%) cultures. Contamination was detected in 12/93 (13%) STC-NRA cultures vs. 29/93 (31.2%) MGIT cultures. The mean time to detection (TTD) of MTC using STC-NRA was 14 days and 7 days using MGIT. CONCLUSION The STC-NRA method is sensitive for the detection of MTC in sputum. TTD increased with duration of anti-tuberculosis treatment, highlighting the value of this method in monitoring treatment success. The method is simple and inexpensive and, unlike MGIT, does not require technical equipment. The preliminary performance characteristics of the method should be further evaluated in larger studies. |
| تدمد: | 1815-7920 1027-3719 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::380cad9bf4c9199b91a98a12e4b39421 https://doi.org/10.5588/ijtld.13.0317 |
| رقم الأكسشن: | edsair.doi...........380cad9bf4c9199b91a98a12e4b39421 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18157920 10273719 |
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