Molecular alterations in theTP53 gene of peripheral blood cells of patients with chronic myeloid leukemia
العنوان: | Molecular alterations in theTP53 gene of peripheral blood cells of patients with chronic myeloid leukemia |
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المؤلفون: | Işık Bökesoy, Rivka Yona, Gurol Tuncman, Ajlan Tükün, Varda Rotter, Miron Prokocimer, Shoshana Peller, Akin Uysal, Yulia Kopilova, Naomi Goldfinger, Halil Gürhan Karabulut |
المصدر: | Genes, Chromosomes and Cancer. 21:2-7 |
بيانات النشر: | Wiley, 1998. |
سنة النشر: | 1998 |
مصطلحات موضوعية: | Cancer Research, Wild type, Intron, Myeloid leukemia, Biology, Molecular biology, Pathogenesis, genomic DNA, Exon, hemic and lymphatic diseases, Immunology, Genetics, Coding region, neoplasms, Gene |
الوصف: | The TP53 gene has been extensively studied in patients with chronic myeloid leukemia (CML), both in chronic phase and in blast crisis. Mutations in the gene were found in up to 30% of the patients, especially among those in blast crisis. We report the results of an analysis of 29 blood samples from CML patients: 8 samples from chronic phase patients, 8 from patients in the accelerated phase, and 13 from patients in blast crisis. By using genomic DNA, we sequenced PCR products of the coding exons and most introns of the TP53 gene, finding genetic changes in 30% of the blast crisis samples and 12% in chronic phase. All mutations were found in introns and were previously unreported. Immunocytochemical studies revealed accumulation of TP53 in blood cells of samples both from chronic phase and blast crisis patients. Since these samples had no TP53 mutations, we believe that wild type TP53 accumulates in blood cells of CML patients. Our results, therefore, indicate that molecular changes in coding regions of the TP53 gene are rare. The significance of the abundance of intronic changes should be investigated further. Accumulation of wild type TP53 in CML cells may indicate an additional mechanism involving this gene in the pathogenesis of this |
تدمد: | 1098-2264 1045-2257 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::3a68356464876dadc620ea37e06038c0 https://doi.org/10.1002/(sici)1098-2264(199801)21:1<2::aid-gcc2>3.0.co;2-5 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi...........3a68356464876dadc620ea37e06038c0 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 10982264 10452257 |
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