Extraction, Fractionation and Characterization of Cotton (Gossypium herbaceum L.) Seed Proteins
| العنوان: | Extraction, Fractionation and Characterization of Cotton (Gossypium herbaceum L.) Seed Proteins |
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| المؤلفون: | Nitesh S Litoriya, Avadh K. Shah, Kelvin Gandhi, Jayantkumar G. Talati |
| المصدر: | Indian Journal of Agricultural Biochemistry. 30:21 |
| بيانات النشر: | Diva Enterprises Private Limited, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0106 biological sciences, biology, Globulin, Chemistry, Sodium, Albumin, chemistry.chemical_element, 04 agricultural and veterinary sciences, Plant Science, Fractionation, biology.organism_classification, 040401 food science, 01 natural sciences, Biochemistry, Gossypium herbaceum, 0404 agricultural biotechnology, Glutelin, Botany, biology.protein, Food science, Prolamin, Agronomy and Crop Science, Polyacrylamide gel electrophoresis, 010606 plant biology & botany |
| الوصف: | Protein fractions (water-soluble/albumin, salt-soluble/globulin, alkali-soluble/glutelin, and alcohol-soluble/prolamin) were extracted from fifteen cotton (Gossypium herbaceum L.) genotypes defatted seed using water, 0.5 M sodium chloride solution, alkali/pH 11.0, and 70% ethanol, sequentially. The main protein fraction was water soluble albumin (40.6 to 56.29%) followed by globulin (21.69 to 42.78%), glutelin (4.8 to 34.7%) and prolamin (0.81 to 2.47%) among the genotypes. Genotype Gv Hv 715 contained high albumin, Bagasara ghed selection contained high glutelin whereas A.D.C-1 contained high globulin and prolamin. The molecular size of the albumin and glutelin fractions was 20 to 55 kDa whereas globulin was 20 to 125 kDa. No prolamin was detected on gel. Cotton seed protein fractions with unique protein profiles could impart characterization of different cotton genotypes. |
| تدمد: | 0974-4479 0970-6399 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::3db91d94c460085567ad3f7ce9e831e2 https://doi.org/10.5958/0974-4479.2017.00003.x |
| رقم الأكسشن: | edsair.doi...........3db91d94c460085567ad3f7ce9e831e2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09744479 09706399 |
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