ADA3: a gene, identified by resistance to GAL4-VP16, with properties similar to and different from those of ADA2
| العنوان: | ADA3: a gene, identified by resistance to GAL4-VP16, with properties similar to and different from those of ADA2 |
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| المؤلفون: | Julie Agapite, Gregory A. Marcus, Benjamin Piña, Neal S. Silverman, Leonard Guarente, Shelley L. Berger |
| المصدر: | Molecular and Cellular Biology. 13:5981-5989 |
| بيانات النشر: | Informa UK Limited, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | SAGA complex, General transcription factor, biology, Transcription (biology), Mutant, biology.protein, Nucleic acid sequence, RNA polymerase II, Promoter, Cell Biology, Molecular Biology, Molecular biology, Gene |
| الوصف: | We describe the isolation of a yeast gene, ADA3, mutations in which prevent the toxicity of GAL4-VP16 in vivo. Toxicity was previously proposed to be due to the trapping of general transcription factors required at RNA polymerase II promoters (S. L. Berger, B. Piña, N. Silverman, G. A. Marcus, J. Agapite, J. L. Regier, S. J. Triezenberg, and L. Guarente, Cell 70:251-265, 1992). trans activation by VP16 as well as the acidic activation domain of GCN4 is reduced in the mutant. Other activation domains, such as those of GAL4 and HAP4, are only slightly affected in the mutant. This spectrum is similar to that observed for mutants with lesions in ADA2, a gene proposed to encode a transcriptional adaptor. The ADA3 gene is not absolutely essential for cell growth, but gene disruption mutants grow slowly and are temperature sensitive. Strains doubly disrupted for ada2 and ada3 grow no more slowly than single mutants, providing further evidence that these genes function in the same pathway. Selection of initiation sites by the general transcriptional machinery in vitro is altered in the ada3 mutant, providing a clue that ADA3 could be a novel general transcription factor involved in the response to acidic activators. |
| تدمد: | 1098-5549 0270-7306 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::3e16b695802dc2d9c08fc1beb4a9fac8 https://doi.org/10.1128/mcb.13.10.5981 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........3e16b695802dc2d9c08fc1beb4a9fac8 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 10985549 02707306 |
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