Ejaculates from peripubertal bulls (n = 468) in 5 different populations were analyzed individually for the presence of fertility- associated antigen (FAA) using a lateral flow cassette. Bull age, scrotal circumference, ejaculate volume, sperm cells per milliliter of ejaculate, percentage of motile sperm, percentage of normal sperm, and number of sperm in the ejaculate were recorded at each collection. These data were compared in bulls with and without FAA in the ejaculate. The percentage of bulls that were FAA-positive (FAA present in the ejaculate) ranged from 65 to 86% among the 5 populations. The only variable that differed across all bulls at first semen collection was ejaculate volume with FAA-negative (FAA not detected in the ejaculate) bulls having a greater volume (P
