The present paper investigates the effects of different nanoparticles blended with biodiesel on performance and emission characteristics of the diesel engine. However, inherent drawbacks like lower heating value, higher density, and viscosity while increased fuel consumption and NOx emissions limit the biodiesel application in engines. In this way, the additives in the form of nanoparticles of different materials play a prominent role to mitigate the drawbacks of biodiesel. The higher calorific value, thermal conductivity, and surface to volume ratio properties of nanoparticles enhanced the engine performance, combustion and emissions compared to neat biodiesel and biodiesel-diesel blends. This study uses five different Diesel, B20, B20CO, B20TO and B20COTO test fuels to be prepared at a different volume mixing ratio. It was concluded that by B20COTO nanoparticle additive fuel showed high BTE and reduced BSFC, HC and NOx emissions on a diesel engine as correlated to other fuels. B20CO fuel showed lower CO emissions as compared other fuels.