Comparative analysis of the bioenergetics of adult cardiomyocytes and nonbeating HL-1 cells: respiratory chain activities, glycolytic enzyme profiles, and metabolic fluxesThis article is one of a selection of papers from the NATO Advanced Research Workshop on Translational Knowledge for Heart Health (published in part 2 of a 2-part Special Issue)
| العنوان: | Comparative analysis of the bioenergetics of adult cardiomyocytes and nonbeating HL-1 cells: respiratory chain activities, glycolytic enzyme profiles, and metabolic fluxesThis article is one of a selection of papers from the NATO Advanced Research Workshop on Translational Knowledge for Heart Health (published in part 2 of a 2-part Special Issue) |
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| المؤلفون: | Andres Piirsoo, Valdur Saks, Kersti Tepp, Lumme Kadaja, Enn SeppetE. Seppet, Nathalie Beraud, Siham ChahbounS. Chahboun, Tuuli Kaambre, Andrey V. Kuznetsov, Mart Roosimaa, Claire Monge, Sophie Pelloux, Yves Tourneur |
| المصدر: | Canadian Journal of Physiology and Pharmacology. 87:318-326 |
| بيانات النشر: | Canadian Science Publishing, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Pharmacology, 0303 health sciences, Hexokinase, biology, Physiology, 030302 biochemistry & molecular biology, Respiratory chain complex, Respiratory chain, General Medicine, Oxidative phosphorylation, Mitochondrion, 03 medical and health sciences, chemistry.chemical_compound, Biochemistry, chemistry, Physiology (medical), biology.protein, Glycolysis, Creatine kinase, Pyruvate kinase, 030304 developmental biology |
| الوصف: | Comparative analysis of the bioenergetic parameters of adult rat cardiomyocytes (CM) and HL-1 cells with very different structure but similar cardiac phenotype was carried out with the aim of revealing the importance of the cell structure for regulation of its energy fluxes. Confocal microscopic analysis showed very different mitochondrial arrangement in these cells. The cytochrome content per milligram of cell protein was decreased in HL-1 cells by a factor of 7 compared with CM. In parallel, the respiratory chain complex activities were decreased by 4–8 times in the HL-1 cells. On the contrary, the activities of glycolytic enzymes, hexokinase (HK), and pyruvate kinase (PK) were increased in HL-1 cells, and these cells effectively transformed glucose into lactate. At the same time, the creatine kinase (CK) activity was significantly decreased in HL-1 cells. In conclusion, the results of this study comply with the assumption that in contrast to CM in which oxidative phosphorylation is a predominant provider of ATP and the CK system is a main carrier of energy from mitochondria to ATPases, in HL-1 cells the energy metabolism is based mostly on the glycolytic reactions coupled to oxidative phosphorylation through HK. |
| تدمد: | 1205-7541 0008-4212 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::4477100ba21fb90daf5aa76b6a21dfed https://doi.org/10.1139/y09-018 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........4477100ba21fb90daf5aa76b6a21dfed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 12057541 00084212 |
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