A nested multiplex PCR for species-specific identification and detection of Botryosphaeriaceae species on mango
| العنوان: | A nested multiplex PCR for species-specific identification and detection of Botryosphaeriaceae species on mango |
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| المؤلفون: | Hong-Ren Yang, Ruey-Fen Liou, Ruey-Shyang Chen, Ting-Hsuan Hung, Hui-Fang Ni |
| المصدر: | European Journal of Plant Pathology. 133:819-828 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | biology, Inoculation, food and beverages, Plant Science, Horticulture, Ribosomal RNA, Fusicoccum aesculi, Botryosphaeriaceae, biology.organism_classification, Multiplex polymerase chain reaction, Botany, Agronomy and Crop Science, Nested polymerase chain reaction, Specific identification, Lasiodiplodia theobromae |
| الوصف: | Lasiodiplodia theobromae (Pat.) Griff. & Maubl, Neofusicoccum parvum Pennycook & Samuels, N. mangiferae Syd. & P. Syd., and Fusicoccum aesculi Corda, all anamorphs of Botryosphaeriaceae species, are the causal agents of mango stem-end rot and fruit rot in Taiwan. Identification of these fungal species based on morphology has not been easy due to their extensive plasticity for some of the morphological characters. To aid reliable identification of Botryosphaeriaceae species associated with mango fruits, four pairs of species-specific primers were designed according to sequences of the ribosomal internal transcribed spacers (ITS), and a rapid method was established based on nested multiplex polymerase chain reaction (PCR) in this study. To perform the analysis, PCR was first run with ITS1 and ITS4 as the primers, followed by a second PCR with the addition of all four sets of species-specific primers. With this method, a low limit of 100 fg-1 pg of purified fungal DNA was detectable. It could also successfully detect L. theobromae, N. parvum, N. mangiferae and F. aesculi in total DNA extracted from inoculated mango fruits. This assay provides a rapid and sensitive method for the identification of Botryosphaeriaceae species and diagnosis of mango fruit rot and stem-end rot as well. |
| تدمد: | 1573-8469 0929-1873 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::458212c11f5a7deddcbc808e72522115 https://doi.org/10.1007/s10658-012-0003-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........458212c11f5a7deddcbc808e72522115 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15738469 09291873 |
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