Reversion of gold nanoparticle aggregates for the detection of Cu2+and its application in immunoassays
| العنوان: | Reversion of gold nanoparticle aggregates for the detection of Cu2+and its application in immunoassays |
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| المؤلفون: | Tzu-Heng Wu, Chie-Pein Chen, Chung-Han Lee, Chen-Yu Chen, Chii-Wann Lin, Chia-Chen Chang |
| المصدر: | The Analyst. 142:4684-4690 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Detection limit, Chromatography, Aqueous solution, medicine.diagnostic_test, Chemistry, 010401 analytical chemistry, Nanoparticle, chemistry.chemical_element, Nanotechnology, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, Biochemistry, Copper, 0104 chemical sciences, Analytical Chemistry, Colloidal gold, Immunoassay, Electrochemistry, medicine, Environmental Chemistry, Reactivity (chemistry), 0210 nano-technology, Spectroscopy, Electrostatic interaction |
| الوصف: | A high concentration of copper is a hazardous element to organisms and human health. Although various strategies have been reported for the sensitive detection of copper, a facile and rapid detection of aqueous copper has seldom been addressed to date. Here, we present an easy and accessible colorimetric method to detect Cu2+ using the redispersion of cysteamine-modified gold nanoparticles (CA-AuNPs). Initially, CA caused the aggregation of AuNPs due to the electrostatic interaction and aggregated AuNPs can be regenerated in basic medium. The subsequent addition of Cu2+ to the CA-AuNP dispersion could effectively trigger the aggregation of CA-AuNPs, resulting from the coordination reactivity between the deprotonated CA and Cu2+. This strategy resulted in a detection limit (LOD) of 1.52 μM in drinking water, which is below the U.S. Environmental Protection Agency permissible limit (20 μM). To demonstrate the broad application of CA-AuNPs, we further applied this method to plasmonic immunoassays based on the competitive interaction of Cu2+ between CA-AuNPs and enzymes. The LOD of the Down syndrome biomarker hyperglycosylated human chorionic gonadotropin (H-hCG) was 0.125 mIU mL−1, which is better than that of commercial immunoassays. Importantly, the determination of H-hCG in serum indicates its applicability for the measurement of real samples. Our assay agrees well with the current immunoassay systems and thus it can easily be expanded to a more common sensing platform for different types of biotargets by changing the corresponding antibodies. |
| تدمد: | 1364-5528 0003-2654 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::48a9b0825d23e5e025723cf279741f21 https://doi.org/10.1039/c7an01511a |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........48a9b0825d23e5e025723cf279741f21 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13645528 00032654 |
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