DNA typing of male-specific polymorphisms is a well-established procedure of molecular analysis. A haplotype of eight different human male Y-specific short tandem repeats (STRs) has been intensively used for forensic casework. This haplotype has also been effectively used to address specific problems of population genetics. A collection of 50 male genomes from our laboratory previously genotyped for 8-Y-STR has been reinvestigated with a battery of eight single nucleotide polymorphisms (SNPs) mapping to the Y-chromosome. The addition of these biallelic markers provided additional identification power. Population investigation revealed genetic structure in Italy, with notably implications in Forensic Genetics. D 2003 Elsevier Science B.V. All rights reserved.