Ternary-column system for high-throughput direct-injection bioanalysis by liquid chromatography/tandem mass spectrometry
| العنوان: | Ternary-column system for high-throughput direct-injection bioanalysis by liquid chromatography/tandem mass spectrometry |
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| المؤلفون: | Daisy B. Whigan, Yuan-Qing Xia, Mohammed Jemal, Mark L. Powell |
| المصدر: | Rapid Communications in Mass Spectrometry. 14:105-111 |
| بيانات النشر: | Wiley, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Bioanalysis, Chromatography, Chemistry, Organic Chemistry, Selected reaction monitoring, Extraction (chemistry), Analytical chemistry, Standard solution, Mass spectrometry, Tandem mass spectrometry, Analytical Chemistry, Standard curve, Liquid chromatography–mass spectrometry, Spectroscopy |
| الوصف: | As a continuation of our efforts to improve our high-flow on-line bioanalytical approach for high-throughput quantitation of drugs and metabolites in biological matrices by high-performance liquid chromatography (LC) and tandem mass spectrometry (MS/MS), we have developed a ternary-column on-line LC/MS/MS system with dual extraction columns used in parallel for purification and an analytical column for analysis. The advantage of the dual extraction column system is that sample analysis can take place in one of the extraction columns while the other column is being equilibrated. Thus, the equilibration time does not add to the run time, hence shortening the injection cycle time and increasing the sample throughput. Moreover, the use of two extraction columns in parallel increases the number of samples that can be injected before the system fails due to an overused extraction column. Such a system has successfully been used to develop and validate a positive ion electrospray LC/MS/MS bioanalytical method for the quantitative determination of a guanidine-containing drug candidate in rat plasma. The system used for this work utilized two Oasis HLB extraction columns (1 x 50 mm, 30 microm), one C18 analytical column (3.9 x 50 mm, 5 microm), a ten-port switching value and a tandem mass spectrometer. The on-line analysis was accomplished by the direct injection of 10 microL of the sample, obtained by mixing a rat plasma sample 1:1 with an aqueous internal standard solution. Selected reaction monitoring (SRM) was utilized for the detection of the analyte and internal standard. The standard curve range was 1.00-200 ng/mL. The intra- and inter-day precision and accuracy were within 6.6%. The on-line purification step lasted for only 0.3 min and total run time was only 1.6 min. |
| تدمد: | 1097-0231 0951-4198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::4d2bad8487a193856e72a1cc36904948 https://doi.org/10.1002/(sici)1097-0231(20000130)14:2<105::aid-rcm842>3.0.co;2-q |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........4d2bad8487a193856e72a1cc36904948 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970231 09514198 |
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